atp biosensor line Search Results


tau rd p301s biosensor hek cell line  (ATCC)
96
ATCC tau rd p301s biosensor hek cell line
Seeding competent Tau-F aggregates are excreted from astrocytes of both genotypes but seeding is more efficient in the presence of ApoE 2 RD tau <t>P301S</t> FRET biosensor cells treated with ACM from Tau-F exposed APOE 2/2 or APOE 4/4 astrocytes. Positive control is the starting medium (same as the astrocytes were exposed to at day 0), and negative control is the astrocyte medium without tau (AM). All medium was fortified with 1% lipofectamine and 10% FBS. (A) Representative YFP images of Biosensor cells after 48h exposure to ACM from the first and last time point (3days+4days ACM, 3days + 12days ACM) plus positive and negative controls. The purple arrows indicate examples of positive puncta. Scale bars = 100 μm. (B) Quantification of YFP IntDen normalized to the cell area. Analyzed using one-way ANOVA with multiple comparisons between APOE 2/2 and APOE 4/4 for each time point. (C) Schematic figure of the experimental layout. Tau-F was diluted in ACM from control astrocytes and added to FRET biosensor cells. (D) Representative YFP images of Biosensor cells exposed to tau diluted in ACM from APOE 2/2 (APOE 2/2 medium) and APOE 4/4 astrocytes (APOE 4/4 medium). Scale bars = 100 μm. (E) Quantification of YFP IntDen, normalized to the cell area. Data are presented as mean ± SD. P-values are presented as following; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.005, ∗∗∗∗ p < 0.0001.
Tau Rd P301s Biosensor Hek Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/atp+biosensor+line/pmc11549983-367-1-8?v=ATCC
Average 96 stars, based on 1 article reviews
tau rd p301s biosensor hek cell line - by Bioz Stars, 2026-07
96/100 stars
  Buy from Supplier
atp biosensor line  (ATeam Scientific)
90
ATeam Scientific atp biosensor line
Seeding competent Tau-F aggregates are excreted from astrocytes of both genotypes but seeding is more efficient in the presence of ApoE 2 RD tau <t>P301S</t> FRET biosensor cells treated with ACM from Tau-F exposed APOE 2/2 or APOE 4/4 astrocytes. Positive control is the starting medium (same as the astrocytes were exposed to at day 0), and negative control is the astrocyte medium without tau (AM). All medium was fortified with 1% lipofectamine and 10% FBS. (A) Representative YFP images of Biosensor cells after 48h exposure to ACM from the first and last time point (3days+4days ACM, 3days + 12days ACM) plus positive and negative controls. The purple arrows indicate examples of positive puncta. Scale bars = 100 μm. (B) Quantification of YFP IntDen normalized to the cell area. Analyzed using one-way ANOVA with multiple comparisons between APOE 2/2 and APOE 4/4 for each time point. (C) Schematic figure of the experimental layout. Tau-F was diluted in ACM from control astrocytes and added to FRET biosensor cells. (D) Representative YFP images of Biosensor cells exposed to tau diluted in ACM from APOE 2/2 (APOE 2/2 medium) and APOE 4/4 astrocytes (APOE 4/4 medium). Scale bars = 100 μm. (E) Quantification of YFP IntDen, normalized to the cell area. Data are presented as mean ± SD. P-values are presented as following; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.005, ∗∗∗∗ p < 0.0001.
Atp Biosensor Line, supplied by ATeam Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/atp+biosensor+line/pm39837326-77-16-19?v=ATeam+Scientific
Average 90 stars, based on 1 article reviews
atp biosensor line - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
fluorescent atp biosensor  (ATeam Scientific)
90
ATeam Scientific fluorescent atp biosensor
Seeding competent Tau-F aggregates are excreted from astrocytes of both genotypes but seeding is more efficient in the presence of ApoE 2 RD tau <t>P301S</t> FRET biosensor cells treated with ACM from Tau-F exposed APOE 2/2 or APOE 4/4 astrocytes. Positive control is the starting medium (same as the astrocytes were exposed to at day 0), and negative control is the astrocyte medium without tau (AM). All medium was fortified with 1% lipofectamine and 10% FBS. (A) Representative YFP images of Biosensor cells after 48h exposure to ACM from the first and last time point (3days+4days ACM, 3days + 12days ACM) plus positive and negative controls. The purple arrows indicate examples of positive puncta. Scale bars = 100 μm. (B) Quantification of YFP IntDen normalized to the cell area. Analyzed using one-way ANOVA with multiple comparisons between APOE 2/2 and APOE 4/4 for each time point. (C) Schematic figure of the experimental layout. Tau-F was diluted in ACM from control astrocytes and added to FRET biosensor cells. (D) Representative YFP images of Biosensor cells exposed to tau diluted in ACM from APOE 2/2 (APOE 2/2 medium) and APOE 4/4 astrocytes (APOE 4/4 medium). Scale bars = 100 μm. (E) Quantification of YFP IntDen, normalized to the cell area. Data are presented as mean ± SD. P-values are presented as following; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.005, ∗∗∗∗ p < 0.0001.
Fluorescent Atp Biosensor, supplied by ATeam Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/atp+biosensor+line/pm30686527-340-5-8?v=ATeam+Scientific
Average 90 stars, based on 1 article reviews
fluorescent atp biosensor - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier

Image Search Results


Seeding competent Tau-F aggregates are excreted from astrocytes of both genotypes but seeding is more efficient in the presence of ApoE 2 RD tau P301S FRET biosensor cells treated with ACM from Tau-F exposed APOE 2/2 or APOE 4/4 astrocytes. Positive control is the starting medium (same as the astrocytes were exposed to at day 0), and negative control is the astrocyte medium without tau (AM). All medium was fortified with 1% lipofectamine and 10% FBS. (A) Representative YFP images of Biosensor cells after 48h exposure to ACM from the first and last time point (3days+4days ACM, 3days + 12days ACM) plus positive and negative controls. The purple arrows indicate examples of positive puncta. Scale bars = 100 μm. (B) Quantification of YFP IntDen normalized to the cell area. Analyzed using one-way ANOVA with multiple comparisons between APOE 2/2 and APOE 4/4 for each time point. (C) Schematic figure of the experimental layout. Tau-F was diluted in ACM from control astrocytes and added to FRET biosensor cells. (D) Representative YFP images of Biosensor cells exposed to tau diluted in ACM from APOE 2/2 (APOE 2/2 medium) and APOE 4/4 astrocytes (APOE 4/4 medium). Scale bars = 100 μm. (E) Quantification of YFP IntDen, normalized to the cell area. Data are presented as mean ± SD. P-values are presented as following; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.005, ∗∗∗∗ p < 0.0001.

Journal: iScience

Article Title: Tau processing and tau-mediated inflammation differ in human APOEε2 and APOEε4 astrocytes

doi: 10.1016/j.isci.2024.111163

Figure Lengend Snippet: Seeding competent Tau-F aggregates are excreted from astrocytes of both genotypes but seeding is more efficient in the presence of ApoE 2 RD tau P301S FRET biosensor cells treated with ACM from Tau-F exposed APOE 2/2 or APOE 4/4 astrocytes. Positive control is the starting medium (same as the astrocytes were exposed to at day 0), and negative control is the astrocyte medium without tau (AM). All medium was fortified with 1% lipofectamine and 10% FBS. (A) Representative YFP images of Biosensor cells after 48h exposure to ACM from the first and last time point (3days+4days ACM, 3days + 12days ACM) plus positive and negative controls. The purple arrows indicate examples of positive puncta. Scale bars = 100 μm. (B) Quantification of YFP IntDen normalized to the cell area. Analyzed using one-way ANOVA with multiple comparisons between APOE 2/2 and APOE 4/4 for each time point. (C) Schematic figure of the experimental layout. Tau-F was diluted in ACM from control astrocytes and added to FRET biosensor cells. (D) Representative YFP images of Biosensor cells exposed to tau diluted in ACM from APOE 2/2 (APOE 2/2 medium) and APOE 4/4 astrocytes (APOE 4/4 medium). Scale bars = 100 μm. (E) Quantification of YFP IntDen, normalized to the cell area. Data are presented as mean ± SD. P-values are presented as following; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.005, ∗∗∗∗ p < 0.0001.

Article Snippet: The tau RD P301S Biosensor HEK cell line (ATCC, CRL-3275) was cultured in DMEM based medium fortified with 10% FBS at 37°C and 5% CO 2 .

Techniques: Positive Control, Negative Control, Control

Journal: iScience

Article Title: Tau processing and tau-mediated inflammation differ in human APOEε2 and APOEε4 astrocytes

doi: 10.1016/j.isci.2024.111163

Figure Lengend Snippet:

Article Snippet: The tau RD P301S Biosensor HEK cell line (ATCC, CRL-3275) was cultured in DMEM based medium fortified with 10% FBS at 37°C and 5% CO 2 .

Techniques: Recombinant, Protease Inhibitor, Lactate Dehydrogenase Assay, ATP Assay, Bicinchoninic Acid Protein Assay, Labeling, Software